Isolation and Characterization of Lignocellulolytic Bacteria from Municipal Solid Waste Landfill for Identification of Potential Hydrolytic Enzyme

The utilization of lignocellulose biomass as an alternative source renewable energy production via green technology is becoming important, and in line with sustainable development goal initiatives. Lignocellulolytic bacteria, such Bacillus spp., can break down by producing hydrolytic enzymes, which are crucial the successful conversion or lignocellulosic material into energy. This information gave rise to this study, where municipal solid waste sediments a sanitary landfill were sampled screened, lignocellulolytic bacteria isolated characterized. Samples taken from four different locations at Pulau Burung site Malaysia. Lignin starch used sources carbon identify potential that exhibit multi-enzymatic activity. growth rate doubling time bacterial isolates lignin criteria for selection. Eleven screened cellulase activity using iodine Congo red dyes. these ranged 0.8 1.7 U/mL. We carried out 16S rRNA gene sequencing phyla selected isolates. Phylogenetic analysis was also conducted based on sequences related species, tree generated Neighbor-Joining method. In proteolyticus, Sanguinis, spizizenii, paramycoides, paranthracis Neobacillus fumarioli identified promising capable expressing enzymes degrading present waste.